GeneSystems spécialiste du diagnostic de bactéries par biologie moléculaire

GeneDisc STEC
& Pathogenic E. coli O157

• WITH HIGHER SPECIFICITY : REDUCE FALSE POSTIVE RESULTS

Some species of Escherichia coli could potentially be pathogenic to humans. Among them, the STEC (aka VTEC) for Shigatoxic Escherichia coli are the E. coli which produce the Shigatoxine (aka Verotoxine). These proteins are described by the stx genes (stx1 gene and/or stx2 gene) and may produce symptoms ranging from simple diarrhoea to hemorrhagic diarrhoea.

• Performing molecular biology assays targeting specific Escherichia coli stx gene enables reliable detection of STEC.

Among the STEC, some bacteria may be lethal to humans, especially infants under the age of  five. Indeed, they not only produce the Shigatoxine (stx gene) but they can attach to the intestine wall because of the protein Intimin (aka Adhesine) coded by the eae gene. The E.coli carrying these 2 genes (stx + eae) have been identified as responsible for Uremic and Haemolytic Syndrome (UHS).  They are called the EHEC for Entero Hemorrhagic Escherichia coli.

EHEC have been described by the EFSA (European Food Safety Association) which has published the Top 5 pathogenic Escherichia coli  identified in food-borne pathogen outbreaks. They are E. coli which carrys the stx and the eae genes among the following serogroups: E. coli O157:H7, E.coli O145, E. coli O111, E. coli O103 and E. coli O26.

• E. coli O157:H7, as the other top 5 E. coli are EHEC, thus pathogenic only if they are carrying the stx and eae genes.

• Targetting the Pathogenic E.coli O157, the GeneDisc® technology reduces the number of false positives (non-pathogenic E.coli).

Pall GeneSystems has developed the first comprehensive molecular diagnostics test that enables 

• Rapid detection of Pathogenic E.coli O157:H7 and STEC non-O157, plus

• Identification of the Top 5 STEC with their virulence factors.

After an enrichment phase of 8 to 16 hours, a GeneDisc STEC indicates the presence of Pathogenic E. Coli (stx & eae) and direct detection of Pathogenic E. coli O157.

A GeneDisc® EHEC then enables confirmation of the serotype of the E. coli among the top 5 if required.

Thanks to the easy-to-use Pall GeneDisc® technology, this rapid test may be done onsite or within service laboratories.
Please contact us for a list of laboratories.

Documentation

Notice_GeneDisc STEC

141 Ko

Press Links

L_Beutin_Validation Study	230 Ko
EFSA VTEC summary	37 Ko
IAFP_Poster_2008	135 Ko